Vitamin E Hplc

HPLC analysis of vitamin A?

chromatographic conditions in the analysis of liquid vitamin A: a) the type of column b) column / C oven temperature) flow d) the type of mobile phase e) of vitamin A is no standard answer / links to research would be greatly appreciated! God bless.

Hope this is what your looking for? a column of type) – stainless steel 4 NovaPak micron C18 column (3.9 mm x 150 mm) b) column / temperature Oven – 25 ° C, provided none / c) flow rate – 1.5 ml / min d) the type of mobile phase – methanol and water, 95:5, v / v, and was degassed by sonication under pressure reduced for 3 minutes before use. e) Vitamin A standard – Do not know what you mean here exactly, the level of preparation? To quote the document " Stock solutions retinols and tocopherols were prepared by dissolving each vitamin in ethanol (HPLC grade) to 349 mmol / l 11 600 l mol /, respectively. Aliquots of these solutions mother were stored at -20 ° C protected from light and used for up to 2 months. The work solutions were prepared weekly by diluting stock solutions in ethanol to obtain concentrations retinol and tocopherol in the range of 0.17 to 4.2 mmol / l and 11.6 to 232 mmol / l, respectively. An ethanol solution containing both retinol acetate (0.91 mmol / l) and tocopherol acetate (106 mmol / l) was used as the internal standard solution. "I assumed they were looking for Retinol analysis here, I apologize if not

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